Effects of dopamine and melatonin treatment on the expression of the genes associated with artificially induced sexual maturation in Japanese eel, Anguilla japonica.

Japanese eel (Anguilla japonica) is a commercially important fish species in Asia. Understanding factors like photoperiod, temperature, and lunar cycles is crucial for successful aquaculture and managing its reproduction. Melatonin and dopamine (DA) are essential for regulating reproduction in vertebrates, including fish. This study investigated the effects of melatonin and DA on the reproductive system of mature male Japanese eels to better understand reproductive regulation in fish. To clarify the effects of these hormones on sexual maturation in eels, a critical stage in the reproductive process, sexual maturation was induced by injecting human chorionic gonadotropin, which stimulates the production of sex hormones. To check the effect of melatonin and DA on sexual maturation, DA, melatonin, and DA + domperidone were intraperitoneally injected into fish from each group (six per treatment) at a dose of 1 mg/kg body weight. The fish were then examined using quantitative RT-PCR by comparing the messenger RNA level of reproduction-related genes (gonadotropin releasing hormone 1; gnrh1, gonadotropin releasing hormone 2; gnrh2, follicle stimulating hormone; fshß, luteinizing hormone; lhß and DA receptor 2b; d2b), involved in the gonadotropic axis in eels, to those that received a control injection. The results indicate significant differences in the expression levels of gnrh1, gnrh2 and d2b in the brain and d2b, fshß, lhß in the pituitary at different stages of sexual maturation. Melatonin appears to enhance the production of sex gonadotropins, whereas DA inhibits them. These findings suggest an interaction between melatonin and DA in regulating reproduction in Japanese eels.

Survey on reference dosimetry practice in Hokuriku region, Japan.

This study aimed to obtain reference dosimetry practices for flattened photon and electron beams. A questionnaire survey on these practices was sent to 37 facilities that performed radiotherapy using these beams in Fukui, Ishikawa, Niigata and Toyama, Japan. The survey comprised equipment (dosemeters, water phantoms, thermometers, barometers and hygrometers) and procedures for reference dosimetry (water used as the phantoms, verification of chamber placement, periodic checks of ion recombination- and polarity-corrections, pre-irradiation and relative humidity monitoring). Responses were received from 19 institutions. In the 19 institutions, a reference-class dosemeter was used for the reference dosimetry. In 6 of the 19 institutions, a 10-year-old or older dosemeter was used. Any barometers, thermometers and hygrometers were not recalibrated. Dosimetry equipment necessitates regular maintenance. Moreover, it is necessary to have a backup dosimetry system in clinics to account for potential malfunctions or instances when the primary system is sent for calibration.

Activation of the growth-IGF-1 axis, but not appetite, is related to high growth performance in juveniles of the Malabar grouper, Epinephelus malabaricus, under isosmotic condition.

Salinity, a determining factor in aquatic environments, influences fish growth. Here, we evaluated the effect of salinity on osmoregulation and growth performance in juveniles of the Malabar grouper, Epinephelus malabaricus, a species of high commercial value in Asian markets; we also identified the salinity that maximized this species' growth rate. Fish were reared at 26 °C and under a 14:10 h photoperiod with a salinity of 5 psu, 11 psu, 22 psu, or 34 psu for 8 weeks. Change in salinity had minimal impact on the plasma Na+ and glucose concentrations, although the Na+/K+-ATPase (nkaα and nkaß) transcript levels in the gills were significantly lower among fish reared at 11 psu salinity. Concomitantly, oxygen consumption was low in fish reared at 11 psu salinity. The feed conversion ratio (FCR) was lower in fish reared at 5 psu and 11 psu salinities than at 22 psu and 34 psu salinities. However, the specific growth rate (SGR) was higher in fish reared at 11 psu salinity. These results suggest that rearing fish at 11 psu salinity would decrease energy consumption for respiration and improve food-conversion efficiency. Among fish reared at 11 psu salinity, the transcript levels of growth hormone (gh) in the pituitary, as well as its receptor (ghr) and insulin-like growth factor I (igf-1) in the liver, were upregulated; these findings suggested stimulation of the growth axis at low salinity. In contrast, there were minimal differences in the transcript levels of neuropeptide Y (npy) and pro-opiomelanocortin (pomc) in the brains of fish reared at any salinity, suggesting that salinity does not affect appetite. Therefore, growth performance is higher in fish reared at 11 psu salinity because of activation of the GH-IGF system, but not appetite, in Malabar grouper juveniles.

Sensitivity of a bone-equivalent polymer gel dosimeter for measuring the dose to bone during radiation therapy.

Treatment planning systems that use the Monte Carlo algorithm can calculate the dose to the medium (Dm) in non-water-equivalent tissues such as bones. However, Dm cannot be verified using actual measurements; therefore, it is necessary to develop tissue-equivalent dosimeters. In this study, we developed a bone-equivalent polymer gel dosimeter (BPGD) that can measure the dose absorbed by the bone and investigated its sensitivity. The BPGDs were prepared by adding 3.0 mol of calcium hydrogen phosphate dihydrate as a component of bone to an improved dose-sensitive polyacrylamide gelatin and tetrakis hydroxymethyl phosphonium chloride (iPAGAT). One day after preparation, the BPGDs were irradiated with a field size of 15 × 15 cm2 using a 10 MV X-ray beam to evaluate the dose sensitivity, dose-rate dependence, and dose-integration dependence. One day after dose exposure, the BPGDs were scanned using a 0.4 T MRI APERTO Eterna (Hitachi, Tokyo, Japan) to obtain R2 values. The difference between the R2 values of 6 Gy and 0 Gy was up to 5 s-1, and the R2 curve plateaued in the high-dose region. Moreover, the BPGD did not depend on the integration of the dose and dose rates. Therefore, the BPGDs that we developed can determine the radiation dose to bones.

Physical and visual evaluations of CT image quality of large low-contrast objects with visual model-based iterative reconstruction technique: a phantom study.

We aimed to verify whether the image quality of large low-contrast objects can be improved using visual model-based iterative reconstruction (VMR) while maintaining the visibility of conventional filtered back projection (FBP) and reducing radiation dose through physical and visual evaluation. A 64-row multi-slice CT system with SCENARIA View (FUJIFILM healthcare Corp. Tokyo, Japan) was used. The noise power spectrum (NPS), task-based transfer function (TTF), and signal-to-noise ratio (SNR) were physically evaluated. A low contrast object as a substitute for a liver mass was visually evaluated. In the noise measurement, STD1 showed an 18% lower noise compared to FBP. STR4 was able to reduce noise by 58% compared to FBP. The NPS of VMR was similar to those of FBP from low to high spatial frequency. The NPS of VMR reconstructions showed a similar variation with frequency as FBP reconstructions. STD1 showed the highest 10% TTF, and higher 10% TTF was observed with lower VMR level. The SNR of VMR was close to that of FBP, and higher SNR was observed with higher VMR level. In the results of the visual evaluation, there was no significant difference in visual evaluation between STD1 and FBP (p = 0.99) and between STD2 and FBP (p = 0.56). We found that the NPS of VMR images was similar to that of FBP images, and it can reduce noise and radiation dose by 25% and 50%, respectively, without decreasing the visual image quality compared to FBP.

A method for patient-specific DVH verification using a high-sampling-rate log file in an Elekta linac.

We have proposed a method for patient-specific dose-volume histogram (DVH) verification using a 40-ms high-sampling-rate log file (HLF) available in an Elekta linac. Ten prostate volumetric-modulated arc therapy plans were randomly selected, and systematic leaf position errors of ±0.2, ±0.4, or ±0.8 mm were added to the 10 plans, thereby producing a total of 70 plans. An RTP file was created by interpolating each leaf position in the HLF to obtain values at each control point, which is subsequently exported to a treatment planning system. The isocenter dose calculated by the HLF-based plan to a phantom (Delta4 Phantom+) was compared to that measured by the diode in the phantom in order to evaluate the accuracy of the HLF-based dose calculation. The D95 of the planning target volume (PTV) was also compared between the HLF-based plans and the original plans with the systematic leaf position errors, the latter being referred to as theory-based plans. Sensitivities of the DVH parameters in the target, the rectum, and the bladder were also calculated with the varied systematic leaf position errors. The relative differences in the isocenter doses between the HLF-based calculations and the measurements among the 70 plans were 0.21% ± 0.67% (SD). The maximum relative differences in PTV D95 between the HLF-based and the theory-based plans among the 70 cases were 0.11%. The patient-specific DVH verification method detected a change in the target DVH parameters of less than 1% when the systematic leaf position error was ±0.2 mm. It is therefore suggested that the proposed DVH verification method may simplify patient-specific dose quality assurance procedures without compromising accuracy and sensitivity.

Transcriptome Analysis in a Scleractinian Coral, Acropora tenuis, during the Spawning Season With Reference to the Gonadal Condition.

Synchronous spawning is a striking feature of coral. Although it is important for reproductive success, corals reallocate energy for reproduction to growth when they are damaged by external stimuli. To assess the transcriptome before and after spawning in the scleractinian coral Acropora tenuis, we tagged three colonies (one bleached and two unbleached) in the field around Sesoko Island (Okinawa, Japan) in November 2016, sampled them monthly from May to July 2017, and performed RNA sequencing (RNA-Seq) analysis. Histological analysis revealed that the previously bleached colony possessed gametes in June, by which time the other two colonies had already spawned. In RNA-Seq analyses, multi-dimensional scaling based on gene expression similarity among the samples reflected the differences between colonies and between months except for the sample of a non-spawned colony in May, which was similar to the samples in June. The similarity of the non-spawned colony sample in May to the samples in June was also shown in hierarchical clustering based on the expression patterns of the genes that were differentially expressed between months in the spawned colonies. These results suggest that non-spawning was already decided in May, and that the physiological condition in a non-spawned colony in May was advanced to June. RNA-Seq analysis also showed that genes related to gametogenesis and those related to apoptosis were upregulated before and after spawning, respectively.

Effect of short- and long-term melatonin treatments on the reproductive activity of the tropical damselfish Chrysiptera cyanea.

Photoperiod plays a role in controlling the initiation and termination of reproduction in fish. Melatonin is an internal transducer of environmental photoperiod and is involved in regulating reproduction. The present study aimed to examine how melatonin impacts the transcript levels of kisspeptin (kiss1 and kiss2), gonadotropin-releasing hormones (gnrh1), and the ß-subunit of gonadotropins (fshß and lhß) in the brain of the sapphire devil, a tropical damselfish with long photoperiod preference. Feeding mature females with melatonin-containing pellets inhibited increases in the transcript levels of kiss1, gnrh1, and lhß within 3 h. Continuous melatonin treatment for 1 week resulted in oocyte regression and downregulation of kiss2, gnrh1, fshß, and lhß. When the transcript levels of kiss1 and gnrh1 were measured at 4-h intervals in the brain of sapphire devil, a day-high/night-low fluctuation was observed. The hypothalamic-pituitary-gonadal (HPG) axis may be influenced by melatonin, exerting a negative effect at night because the transcript levels of aralkylamine N-acetyltransferase (aanat2) increased during the scotophase. The expression of aanat2 was higher under short-day than long-day conditions, suggesting that there is a seasonal change in melatonin levels at night. It was concluded that change in photoperiod becomes a key factor for controlling the hormone synthesis in the HPG axis through melatonin.

Accuracy of patient setup positioning using surface-guided radiotherapy with deformable registration in cases of surface deformation.

The Catalyst™ HD (C-RAD Positioning AB, Uppsala, Sweden) is surface-guided radiotherapy (SGRT) equipment that adopts a deformable model. The challenge in applying the SGRT system is accurately correcting the setup error using a deformable model when the body of the patient is deformed. This study evaluated the effect of breast deformation on the accuracy of the setup correction of the SGRT system. Physical breast phantoms were used to investigate the relationship between the mean deviation setup error obtained from the SGRT system and the breast deformation. Physical breast phantoms were used to simulate extension and shrinkage deformation (-30 to 30 mm) by changing breast pieces. Three-dimensional (3D) Slicer software was used to evaluate the deformation. The maximum deformations in X, Y, and Z directions were obtained as the differences between the original and deformed breasts. We collected the mean deviation setup error from the SGRT system by replacing the original breast part with the deformed breast part. The mean absolute difference of lateral, longitudinal, vertical, pitch, roll, and yaw, between the rigid and deformable registrations was 2.4 ± 1.7 mm, 1.3 ± 1.2 mm, 6.4 ± 5.2 mm, 2.5° ± 2.5°, 2.2° ± 2.4°, and 1.0° ± 1.0°, respectively. Deformation in the Y direction had the best correlation with the mean deviation translation error (R = 0.949) and rotation error (R = 0.832). As the magnitude of breast deformation increased, both mean deviation setup errors increased, and there was greater error in translation than in rotation. Large deformation of the breast surface affects the setup correction. Deformation in the Y direction most affects translation and rotation errors.

Ovarian Development and Larval Release of the Hermit Crab Clibanarius eurysternus (Hilgendorf, 1879) (Decapoda: Anomura: Diogenidae) in Okinawa, Japan.

This study investigated ovarian development and larval release in the hermit crab Clibanarius eurysternus, which inhabits the upper intertidal zone on Okinawa, Japan. Adult females were collected monthly and had a high gonadosomatic index (GSI) from May to October. Oocytes at vitellogenic stages were observed from May to October. Some females had post-ovulatory follicles in the ovary and embryos on the abdomen (ovigerous females) in June and August, suggesting active ovarian development and larval release during these months (breeding season). When adult females were collected weekly according to the lunar cycle, a high GSI with oocytes at the tertiary yolk globule stage in the ovaries was maintained in all lunar phases. Since post-ovulated follicles were seen in ovaries collected around the last quarter and new moons, darkness at night is needed for spawning. Ovigerous females exposed to stepwise changes in hydrostatic pressure exhibited significantly higher synchrony between larval release and high pressure at night from the last quarter moon to the new moon, suggesting the importance of the tidal amplitude cycle in larval release under non-moonlight conditions. We concluded that the continuity of synchronous spawning and larval release of C. eurysternus are repeated under darkness and high tide at night during the breeding season.

Testis development in the Japanese eel is affected by photic signals through melatonin secretion.

OBJECTIVE: According to reported spawning characteristics of Japanese eel, Anguilla japonica, which exhibit spawning and migration patterns that are synchronized with lunar cycles and photoperiod, we hypothesized that a close association exists between specific photic signals (daylight, daylength, and moonlight) and endocrinological regulation. Given the photic control in melatonin secretion, this hypothesis was tested by investigating whether melatonin signals act as mediators relaying photic signals during testis development in the eel. METHODS: We examined changes in melatonin-secretion patterns using time-resolved fluorescence immunoassays in sexually immature and mature male Japanese eels under the condition of a new moon (NM) and a full moon (FM). RESULTS: The eye and plasma melatonin levels exhibited a nocturnal pattern under a 12-h light: dark cycle (12L12D) or under constant darkness (DD), but not with constant light (LL). Eye melatonin levels were similar under the 12L12D and short-day (9L15D) conditions. In the long-day condition (15L9D), secreted plasma melatonin levels were stable, whereas short-day melatonin secretion began when darkness commenced. Sexual maturation began at 8 weeks following intraperitoneal injection of human chorionic gonadotropin (hCG), and NM exposure led to significantly higher eye and plasma melatonin levels compared with those detected under FM exposure.

[A Simple Measurement Method for Verifying the Accuracy of the Displayed Dose on an Over-couch-type X-ray Fluoroscopic System].

The purpose of this study was to establish a simple measurement method to verify the accuracy of incident air kerma (Ka, r) and air kerma area product (PKA) displayed on an over-couch-type X-ray fluoroscopy system. A dosimeter was located at the patient entrance reference point, and the irradiation field size was set to 10×10 cm. A lead plate was placed on the couchtop to protect the image receptor, and the duration of fluoroscopy was set to 1 min. The Ka, r was measured with the proposed method and the Japanese Industrial Standards (JIS) method on three X-ray fluoroscopy units of different manufactures. The effect of backscattered X-rays from the lead plate was calculated using Monte Carlo methods. The errors of the displayed Ka, r and PKA to the measured Ka, r and PKA with our proposed method were calculated. There was no significant difference in the measured Ka, r between the proposed method and the JIS method in all units. The effect of backscattered X-ray was ≤0.5%. The errors of displayed Ka, r and PKA to those measured were in the range of 3.4 to 15.7% and -4.1 to 20.3%, respectively, which met the tolerance for accuracy of ±35% in accordance with the JIS method. We found that our proposed method was simple and that the accuracy of measured values was comparable to that of the JIS method.

Use of optically stimulated luminescence dosimeter and radiophotoliminescent glass dosimeter for dose measurement in dual-source dual-energy computed tomography.

We aimed to evaluate properties of optically stimulated luminescence dosimeters (OSLDs) and radiophotoluminescent glass dosimeters (RPLDs) used in dual-source dual-energy (DE) computed tomography (DECT) dosimetry. Energy dependence was evaluated in single-energy (SE) and DE modes, and their relative dose responses differed by 3.8% and 6.6% under equivalent effective energy with OSLD and RPLD, respectively. Dose variation was evaluated using coefficients of variation of dose values from 10 dosimeters, and dose variation of OSLD and RPLD in SE mode ranged from 2.1 to 3.0% and from 2.1 to 2.8%, and those in the DE mode were 1.8 and 2.6%, respectively. Dose linearity was evaluated from 1 to 150 mGy, and linear relationships of dose response were observed between the dosimeters and the ionization chamber (correlation coefficients ≥ 0.9991). Angular dependence was evaluated from - 90° to + 90°, and it was smaller in DE mode than in SE mode for OSLD. The normalized response of RPLD was higher at ± 30° and ± 60° and lower at - 90° in SE and DE modes. This study demonstrated both OSLD and RPLD can perform dosimetry in dual-source DECT with small influence of the properties of the dosimeters compared with that in SECT.

[Impact of Respiratory Motion on Point Doses for Three Whole-breast Irradiation Techniques after Breast-conserving Surgery].

PURPOSE: For whole-breast irradiation after breast-conserving surgery, computed tomography simulation (CTS) and irradiation are generally performed during free breathing. In treatment planning, there are three techniques: field-in-field (FIF), physical wedge (PW), and enhanced dynamic wedge (EDW). The aim of this study was to investigate the impact of respiratory motion on doses for these three irradiation techniques. METHODS: All doses were measured using an ionization chamber in a cylindrical phantom on a respiratory motion platform. Doses for each technique were measured with and without phantom motion. The dose without phantom motion was defined as the reference. The reference was compared to the dose with the phantom motion. The positions of the isocenter with respect to the ranges of phantom motion were set as exhale and intermediate. The phantom motion amplitude was set to 5 mm or 10 mm. The respiratory phase to initiate irradiation was varied as inhale, intermediate-inhale, exhale and intermediate-exhale. RESULTS: When the motion amplitude was 10 mm, the dose differences for the FIF, PW, and EDW techniques were 4.2%, 0.5%, and 0.8%, respectively, at the maximum. However, the dose difference for the FIF technique was -0.5% when the isocenter position was set to the intermediate phase of phantom motion. CONCLUSION: We found that the dose difference per fraction was reduced when the respiratory phase during CTS image acquisition was set to the intermediate phase. Meanwhile, the dose differences per fraction for the PW and EDW techniques were less affected by the respiratory motion.

Evaluation of the relationship between phantom position and computed tomography dose index in cone beam computed tomography when assuming breast irradiation.

This study aims to investigate the influence of the phantom position on weighted computed tomography dose index (CTDIw ) in cone beam computed tomography (CBCT) when assuming breast irradiation. Computed tomography dose index (CTDI) was measured by the x-ray volume imaging of CBCT using parameters for image-guided radiation therapy (IGRT) in right breast irradiation. The measurement points of CTDI ranged from 0 (center) to 16 cm in the right-left (RL) direction, and from 0 (center) to 7.5 cm in the anterior-posterior (AP) direction, which assumed right breast irradiation. A nonuniform change exists in the relative value of CTDIw when the phantom deviated from the isocenter of CBCT. The CTDIw was ~30% lower compared with the value at the isocenter of CBCT when the phantom deviated 7.5 and 16 cm at the AP and RL directions, respectively. This study confirmed the influence of the phantom position on the CTDI values of CBCT. The CTDI measured at the isocenter of CBCT overestimates that measured at the irradiation center of the breast.

Does estrogen regulate vitellogenin synthesis in corals?

Most broadcast spawner corals have a vitellogenic phase that lasts at least 6 months. It is established that estrogen regulates vitellogenin synthesis in vertebrates. Although some research have been conducted on the physiological role of sex steroids in corals, little is known about their involvement in oocyte development. This study aimed to detect steroid hormones - progesterone, testosterone, and estradiol-17ß (E2) - in Acropora tenuis and study the relationships between vitellogenesis/vitellogenin synthesis and these steroids. This study also investigated the effect of E2 on vitellogenin synthesis in corals and identified steroidogenic enzymes in A. tenuis genome. Branches from tagged coral colonies were collected monthly from March to November. Histological observations showed that oocytes were vitellogenic from March to May (Stage IV and V), but not in June, and that gonads were occupied by immature oocytes in September (Stage I). Real-time qPCR revealed that vitellogenin (vg1 and vg2) transcript levels in coral branches were high in April and May, implying that corals actively underwent vitellogenesis during these months, and spawned before June. Liquid chromatography-mass spectrometry revealed that E2 could be detected in coral branches in March, April, and May, but not in June, whereas testosterone and progesterone did not fluctuate much in the same months. Immersing branches in E2-containing seawater failed to increase vitellogenin transcript levels. The results indicate that E2 is involved in oogenesis but does not positively regulate vitellogenin synthesis. Steroidogenic enzymes (except CYP19A) were identified in A. tenuis, suggesting that corals may endogenously synthesize progestogens and androgens from cholesterol.

Effect of an integral quality monitor on 4-, 6-, 10-MV, and 6-MV flattening filter-free photon beams.

PURPOSE: To investigate the effect of an integral quality monitor (IQM; iRT Systems GmbH, Koblenz, Germany) on 4, 6, 10, and 6-MV flattening filter-free (FFF) photon beams. METHODS: We assessed surface dose, PDD20,10 , TPR20,10 , PDD curves, inline and crossline profiles, transmission factor, and output factor with and without the IQM. PDD, transmission factor, and output factor were measured for square fields of 3, 5, 10, 15, 20, 25, and 30 cm and profiles were performed for square fields of 3, 5, 10, 20, and 30 cm at 5-, 10-, and 30-cm depth. RESULTS: The differences in surface dose of all energies for square fields of 3, 5, 10, 15, 20, and 25 cm were within 3.7% whereas for a square field of 30 cm, they were 4.6%, 6.8%, 6.7%, and 8.7% for 4-MV, 6-MV, 6-MV-FFF, and 10-MV, respectively. Differences in PDD20,10 , TPR20,10 , PDD, profiles, and output factors were within ±1%. Local and global gamma values (2%/2 mm) were below 1 for PDD beyond dmax and inline/crossline profiles in the central beam region, respectively. The gamma passing rates (10% threshold) for PDD curves and profiles were above 95% at 2%/2 mm. The transmission factors for 4-MV, 6-MV, 6-MV-FFF, and 10-MV for field sizes from 3 × 3 to 30 × 30 cm2 were 0.926-0.933, 0.937-0.941, 0.937-0.939, and 0.949-0.953, respectively. CONCLUSIONS: The influence of the IQM on the beam quality (in particular 4-MV X-ray has not verified before) was tested and introduced a slight beam perturbation at the surface and build-up region and the edge of the crossline/inline profiles. To use IQM in pre- and intra-treatment quality assurance, a tray factor should be put into treatment planning systems for the dose calculation for the 4-, 6-, 10-, and 6-MV flattening filter-free photon beams to compensate the beam attenuation of the IQM detector.

Evaluation of technical performance of optical surface imaging system using conventional and novel stereotactic radiosurgery algorithms.

The Catalyst HD (C-RAD Positioning AB, Uppsala, Sweden) optical surface imaging (OSI) system is able to manage interfractional patient positioning, intrafractional motion monitoring, and non-contact respiratory gating without x-ray exposure for radiation therapy. In recent years, a novel high-precision surface registration algorithm for stereotactic radiosurgery (SRS algorithm) has been released. This study aimed to evaluate the technical performance of the OSI system using rigid phantoms, by comparing the conventional and SRS algorithms. To determine the system's technical performance, isocenter displacements were calculated by surface image registration via the OSI system using head, thorax, and pelvis rigid phantoms. The reproducibility of positioning was evaluated by the mean value calculated by repeating the registration 10 times, without moving each phantom. The accuracy of positioning was evaluated by the mean value of the residual error, where the 10 offset values given to each phantom were subtracted from the isocenter displacement values. The stability of motion monitoring was evaluated by measuring isocenter drift during 20 min and averaging it over 10 measurements. For the head phantom, all tests were compared with the mask types and algorithms. As a result, for all sites and both algorithms, the reproducibility, accuracy, and stability for translation and rotation were <0.1 mm and <0.1°, <1.0 mm and <1.0°, and <0.1 mm and <0.1°, respectively. In particular, the SRS algorithm had a small absolute error and standard deviation of calculated isocenter displacement, and a significantly higher reproducibility and accuracy than the conventional algorithm (P < 0.01). There was no difference in the stability between the algorithms (P = 0.0280). The SRS algorithm was found to be suitable for the treatment of rigid body sites with less deformation and small area, such as the head and face.

[Estimation of Dynamic MLC Parameters Using Dynamic MLC Position Accuracy Analysis Software in Prostate IMRT].

It is necessary to verify an intensity-modulated radiation therapy (IMRT) plan and to confirm dose error within the tolerance, in order to perform it securely and precisely. IMRT with dynamic multi-leaf collimator (DMLC) requires high DMLC position accuracy. The DMLC position accuracy analysis software DynaLog File Viewer (DFV; Varian Medical Systems, Palo Alto, CA, USA) is used to analyze position errors of DMLC for IMRT plans. We analyzed correlation between DMLC parameters and position error of DMLC obtained from DFV in prostate IMRT. A regression analysis of the position error and the DMLC parameters was performed. As a result, a strong correlation was found between MLC position error and each of the DMLC parameters: leaf speed, gap width, and segment monitor unit (MU). We found the factors for the DMLC position error in this study. DMLC position error could be estimated from leaf speed, gap width, and segment MU when we analyze IMRT cases in the further study.

Food availability alters expression profiles of genes in relation to reproduction and nutrition in the females of tropical damselfish (Chrysiptera cyanea).

This study evaluated the effects of food availability on the transcript levels of genes related to reproduction and growth in the sapphire devil (Chrysiptera cyanea), a tropical damselfish. Nonbreeding fish were reared at high-food (HF) and low-food (LF) levels for 4 weeks under long-days. Vitellogenic oocytes could be observed in the ovaries of the HF group. The quantitative polymerase chain reaction analysis revealed that lhß and cyp19b in the brains, vtg and igf1 in the livers and cyp19a in the ovaries of HF fish were significantly higher than that of LF fish, suggesting that estradiol-17ß (E2) synthesis in the ovary and brain is activated when suitable permissive factors are available to fish. Food limitation lowered hepatic igf1 and dio2, suggesting that the TH-IGF1 signaling system functions in the liver, and that food availability altered hepatic deiodination activities related to intercellular levels of thyroid hormones. Hepatic dio2 significantly decreased when fish were immersed for 3 days in E2-containing seawater; this suggests that E2 impedes the conversion of T4 to T3 in the liver. Our study shows that igf1 was upregulated in accordance with HF-induced vitellogenesis but downregulated by E2 treatment, suggesting that igf1 is bidirectional and altered by maturational status. Once vitellogenesis begins under a suitable range of proximal factors, fish need to maintain their nutritional status because food availability is a permissive factor for their reproduction.